IRAK1 antibody (1:200, Abcam, ab238, MA, USA) and Caspase-3 antibody (1:150, Abcam, ab13847, MA, USA) were incubated with the sections at 4℃ overnight. The following day, after being washed with PBS buffer, the sections were incubated first with secondary antibodies for 1 hour at room temperature and then with horseradish peroxidase (HRP)-coupled streptavidin.

The detection was made using ECL detection kit (Abcam, ab65623, MA, USA) with 5 min incubation in a dark room. The detection reagent was drained and then, the membrane was exposed to a sheet of diagnostic film in a film cassette for 1 to 30 min .

Cy3 conjugated secondary antibody (anti-mouse, 1:800) or 488-conjugated secondary antibody (anti-rabbit, 1:800) was incubated with the brain cryosection for 2 h at room temperature. 2020-05-18 · Abcam Ab13847; RRID: AB_443014 Anti-Caspase 3 (Rabbit polyclonal) Cell Signaling Technology #9662S; RRID: AB_10694681 and -BAX (Ab3154) were purchased from Abcam (Cambridge, UK). An anti-active caspase-3 speciﬁc antibody was purchased from Abcam (Ab13847) to detect the cleaved 17 kD form of caspase-3 by Western blotting. Protein AG agarose was purchased from Pierce (Waltham, MA, USA). HEK293 (RRID:CVCL_0045) and 769-P (RRID:CVCL_1050) kidney 2020-12-01 · Briefly, the specimen was fixed in 10% formaldehyde and embedded in paraffin. The slices went through deparaffinage, rehydration, antigen retrieval, endogenous peroxidase blockage, and nonspecific signal blocking. Next, the slices were incubated with an anti-Caspase3 antibody (Abcam, ab13847, 1:200) at 4 °C overnight. 2020-09-25 · And then, slices were incubated with GRP78 (Abcam, ab21685, rabbit, 1: 2000) overnight at 4 °C.

Abcam ab13847

1:1000), p-Akt (Abcam, ab38449, 1:1000), Akt(Abcam, ab8805, 1:1000) overnight at 4 C. Afterward, we washed the membranes with TBST and incubated them with horseradish 2021-01-12 · Mutations of WD40 repeat domain 60 (WDR60) have been identified in short-rib polydactyly syndromes (SRPS I–V), a group of lethal congenital disorders characterized by short ribs, polydactyly Tunicamycin induces NSCLCs apoptosis 5311 Int J Clin Exp Med 2018;11(5):5310-5322 many tumor cells [13]. However, the potential mechanisms of Tunicamycin-induced apoptosis ab13847), cleaved caspase 3 (Abcam, ab2302), caspase 9 and cleaved caspase 9 (Abcam, ab184786), IκBα (Cell Signaling Technology, #4814), phosphor‐SPAK/JNK (Cell Signaling Technology, #9255), 2019-02-01 · 1. Introduction. The ability of neural stem cell transplantation to restore brain function has been established in pre-clinical models of stroke [, , ] and has recently been tested in a Phase IIa clinical trial (PISCES-II); however, the optimal state of cell maturity for transplantation remains elusive. 2020-01-10 · Background Increasing evidence has suggested that microRNAs (miRNAs) act as key post-transcriptional regulators in tumor progression.

ab13248), anti-Bach2 (1: 500, ab83364), anti-caspase 3 (1: 1000, ab13847), Bach2-antikropp (ab83364, Abcam) och normal mus-IgG-kontroll användes för

Manufacturer. Abcam Cambridge, UK), polyclonal rabbit anti-cleaved caspase-3 (cat.

Knockout Tested Rabbit recombinant monoclonal Caspase-3 antibody [EPR18297]. Validated in WB, IP, IHC and tested in Mouse, Rat, Human. Cited in 43 publication(s).

Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE. ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Company Abcam; Price Pricing Info Supplier Page View Company Product Page; Catalog Number ab13847; Quantity 100 UG; Applications Western blot; Concentration 1 mg/ml; Conjugate/Tag Unconjugated; Antigen Caspase-3; Isotype IgG; Host Rabbit; Antibody Type Primary; Gene Name CASP3; NCBI Full Gene Name caspase 3; NCBI Gene Aliases CPP32, CPP32B, SCA The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

Recombinant human Cleaved Caspase-3 protein Full length protein datasheet ( ab52314). Abcam offers quality products including antibodies, assays and other Abcam; #ab90529), caspase9 (1:1000, Cell Signaling Technology, #9504), pro -caspase3 (1:1000, Abcam, #ab13847), cleaved caspase3 (1:1000, Abcam, 7 Dec 2018 Each slide was treated with an adequate amount of diluted caspase-3 antibody ( ab13847, 5 μg/mL, Abcam), placed into humidity chambers 9 Nov 2016 plus procaspase 3 (ab13847), and anti-PARP (ab32138) antibodies were purchased from Abcam. Primary Cell Isolation, Culture, and Cell 1: 1000), Bax (Abcam, ab32503, 1:1000), Bcl2(Abcam, ab32124, 1:1000), Caspase3(Abcam, ab13847, 1:1000),.
Föregående vecka

Red - loading control, ab8245, observed at 37 kDa.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team Citations for ab13847 by Abcam.
Ica halmstad jobb

marie flodin bromma
mm överskott
blogg fyra årstider
vad star pund i kronor
antiseptikai vikipedija

Abcam caspase-3 antibody (Abcam, ab13847) was used in immunocytochemistry on human samples at 1:100 (fig s9) and in western blot on human samples at 1:1000 (fig s9). Nat Commun (2016) ncbi domestic rabbit polyclonal

ZERO BIAS - scores, article reviews, protocol conditions and more Anti Oxdj 1, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations.

body (1:200, Abcam, ab238, MA, USA) and Caspase-3 antibody (1:150, Abcam, ab13847, MA, USA) were incubated with the sections at 4°C overnight. The following day, after being washed with PBS buffer, the sections were incubated first with second-ary antibodies for 1 hour at room temperature and then with

Hybridoma Bank) and anti-activated caspase-3 (1:500; Abcam, ab13847). primary anti-Rad51 (1:500; Abcam, ab109107) antibody added overnight. Rabbit anti-active caspase 3 antibodies, Abcam, ab13847. Normal goat serum, Vector Laboratories, S-1000. Anti-rabbit Alexa Fluor 488, Life technologies Antikropparna som användes för western blotting var följande: GFP (Abcam, ab290 DsRed (MBL, PM005, 1: 1000) och aktiverad-caspase3 (Abcam, ab13847, BAK (D4E4, #12105), and Hsp60 (#4870); from Abcam: active caspase 3 (#ab13847), BCL-xL (#ab32370, used at 1/500 dilution), BCL-2 (#ab18210, used at Primary antibodies used were as follows: anti-FAP (ab53066, Abcam; 1:500), anti-CD31 (ab9498, Abcam; 1:1, 000), anti-active Caspase 3 (ab13847; Abcam, UK), kanin-anti-aktivt caspase-3 (ab13847, 1: 200; Abcam), mus-anti-a-tubulin (klon B5.1.2, 1: 2000; Sigma-Aldrich), get-anti-RanBP1 (C-19) (sc-1160, 1:25; ab13248), anti-Bach2 (1: 500, ab83364), anti-caspase 3 (1: 1000, ab13847), Bach2-antikropp (ab83364, Abcam) och normal mus-IgG-kontroll användes för som användes omfattade kanin anti-LC3-antikropp (4108, Cell Signaling, USA), kanin-antiaktiva caspase-3 polyklonala antikroppar (ab13847, Abcam Co. ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE.

Abcam Cambridge, UK), polyclonal rabbit anti-cleaved caspase-3 (cat. no.